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Sarah,
Is this what you are looking to do?
http://www.mvia.com/ExtFocus/ext_focus.htm
Please note the "Rotational 3D" movies which show the 3D information is
retained. I realize the extended focus images are not what you are looking for,
but the Rotational 3D images seem to be exactly what you are looking to do. You
can easily rotate your image stack through any angle you desire in the X, Y ,
and Z axes.
Let me know if this is what you're looking for. Please feel free to call or
email me if you have nay questions.
Thanks!
Jim Haley
******************************
Jim Haley
Applications Engineer
MVIA, Inc.
125 Sherwood Drive
Monaca, PA 15061
voice: (724) 728-7493
fax: (412) 291-1709
e-mail: [log in to unmask]
webpage: http://www.mvia.com
******************************
Locknar, Sarah A wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> OK, I guess my first message was not clear. The data is several
> z-stacks that we want to align in x, y, and z. Currently she makes
> projections of each (losing the z-information) and makes a montage in
> photoshop. We're looking for a way to PRESERVE the z-information so the
> entire combined data set can be rotated in 3-dimensions. I guess this
> would be the equivalent of "stitching" in 3-D. Does that help? Any new
> ideas?
> Thanks again-
> Sarah
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Joel Sheffield
> Sent: Monday, May 10, 2004 4:52 PM
> To: [log in to unmask]
> Subject: Re: 3D montage
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> It sounds to me as if you want to make a 3d rendering of a stack. In
> ImageJ, and several other image processing programs, it is possible to
> set a level of transparency and then "stack" the images in such a way
> that the composite can be rotated. There are several levels of this
> kind of processing. You can construct what is conceptually a set of
> plexiglas sheets each of which has an image on it. Then, you can take
> this "stacked" structure and rotate it.
>
> Alternatively, you can select some level of intensity, and draw
> polygons that connect all areas in the stack, and then fill in the
> polygons to create a surface. This process is generally called Volume
> rendering. Again, there is a plugin in Imagej that does a remarkable
> job of this.
>
> Joel Sheffield
>
>
>>Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-
>>bin/wa?S1=confocal Hi Sarah, I"m not clear what is being asked
>>here.The original meaning of "montage" meant showing an image stack
>>presented in a series of rows and/or columns, yes? In that case,
>>MetaMorph and ImageJ do this readily. Compix, on the other hand, uses
>>the term to mean a projection, or making a single imageby combingall
>>the images in the stack.Are you asking can one make a montage of
>>several image stacks, with each stack representing one frame in the
>>montage? Thanks, Carl
>>
>>
>>
>>Carl Boswell
>>Dept. of Molecular and Cellular Biology
>>Univ. of Arizona
>>520-626-8469
>>520-621-3709 FAX
>> ----- Original Message -----
>>From: Locknar, Sarah A
>>To: [log in to unmask]
>>Sent: Monday, May 10, 2004 12:15 PM
>>Subject: 3D montage
>>
>>Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-
>>bin/wa?S1=confocal Hi- Does anyone know of a program that will create
>>a 3D montage of image stacks? The only thing I can think of right now
>>is for the user to create montages in photoshop, save each montage
>>slice as a tiff and then open them as a stack in some 3D rendering
>>program. Any ideas about an easier way to do this? Thanks- Sarah
>>
>>----------------------------------------------------------------------
>>----------------- Sarah Locknar, Ph.D. Director, Neuroscience COBRE
>>Imaging / Physiology Core College of Medicine, University of Vermont
>>E015 Given Building 89 Beaumont Ave. Burlington, VT 05405 802-656-0413
>
>
>>802-656-8704 (fax)
>>----------------------------------------------------------------------
>>-----------------
>>
>
>
>
> Joel B. Sheffield, Ph.D.
> Biology Department, Temple University
> 1900 North 12th Street
> Philadelphia, PA 19122
> [log in to unmask]
> (215) 204 8839, fax (215) 204 0486
> http://astro.temple.edu/~jbs
>
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