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Hi Nuno,
Have you made any comparisons between that and a comparable oil  
immersion objective for live or fixed thick tissues or brain slices?     
Yes, the prices are steep, the Zeiss 100/gly costs far more than any  
car I've ever owned.

Regards,
Glen
On May 17, 2004, at 9:56 AM, Nuno Moreno wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Glen,
>
> I've been using a Leica 64X 1.3 that uses glycerol @ 80% with a
> correction collar from 0.14 to 0.20 mm. Its a very nice objective with  
> a
> good spectral response (At least I'm using it in 2P with good results).
>
> The only problem is the price! I've ordered together with a microscope
> and no one noticed ;)
>
> All the best,
> Nuno Moreno
>
>
>
>
>
>
> Glen MacDonald wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> On the topic of immersion objectives, has anyone experience with high
>> magnification glycerol immersion objectives, in contrast to
>> multi-immersion objectives?  the MI units produce beautiful images but
>> are only available at a max mag. of 25X (at least from Zeiss).  Has
>> anyone used the Zeiss 100X/NA1.2 glycerol objective?  given that the  
>> RI
>> of brain tissue is closer to glycerol, glycerol immersion seems a more
>> reasonable approach than water immersion. A higher mag than 25X would
>> be useful for widefield systems using a CCD camera.  A 63X oil
>> immersion with a coverslip correction collar might be a more practical
>> and affordable alternative, if it existed.
>>
>> Regards,
>> Glen
>>
>>
>> Glen MacDonald
>> Core for Communication Research
>> Virginia Merrill Bloedel Hearing Research Center
>> Box 357923
>> University of Washington
>> Seattle, WA 98195-7923  USA
>> (206) 616-4156
>> [log in to unmask]
>>
>> ********************************************************************** 
>> **
>> ******
>> The box said "Requires Windows 95 or better", so I bought a Macintosh.
>> ********************************************************************** 
>> **
>> ******
>>
>
> --
> ______________________________
> Nuno Moreno
> Phone: +351 214464606
> Fax  : +351 214407970
> www.igc.gulbenkian.pt
> Cell Imaging Unit
> Instituto Gulbenkian de Ciência
> Portugal
> _______________________________
>
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[log in to unmask]

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******
The box said "Requires Windows 95 or better", so I bought a Macintosh.
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