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Dear List Members,

the discussion on FocalClear and MountClear reminds me that this
issue was already discussed  by  this list a year ago, or more, and
that somebody stated that tissue clearing up to 500 um could equally
well be obtained with some standard reagents. I would appreciate
receiving comments and protocols. Moreover, in your experience, how
much thick a PFA-fixed brain slices can be, to still be  permeable to
antibodies for immunocytochemistry?

Regards,
Franco
--
Franco Mangia
Department of Psychology
Section of Neuroscience
University La Sapienza of Rome
Via dei Marsi 78
00185 Rome, Italy
Phone(Office): +39 06 4991 7784
Phone (Lab): +39 06 4991 7868/7869
Preferred Fax: +39 06 4991 7872
Department Fax: +39 06 445 1667
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