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Hi Shekar,
In the absence of genetic contructs or antibodies directed at your specimen,
the next best thing is lectins; molecules that detect relatively specific
carbohydrate combinations on glyco-molecules found on the surface of pertty
much everything.  It would require doing a screening of several lectins to
find something that works, but they can be very selective.  Fluorescently
tagged lectins can be had from a number of vendors, including Sigma Chemical
and Molecular Probes.

good luck,
Carl


Carl Boswell
Dept. of Molecular and Cellular Biology
Univ. of Arizona
520-626-8469
520-621-3709 FAX
----- Original Message -----
From: "Hunthrike Shekar Shetty" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, May 21, 2004 2:34 AM
Subject: Re: Immobilizing bugs


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Hello,
Can you suggest any dye that can be used in Confocal laser scanning
microscoy to stain a  fungi especially for oomycytes, since we do not have a
GFP isolate.
Regards
Shekar Shetty

>>> [log in to unmask] 05/20/04 9:22 PM >>>
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Renato-
There is a product we use to slow down Paramecium.  It is called
"Detain" and it is manufactured by WNSE.  That is all the information
I can get from the label.
Carol H.


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello
>I have a collaborator that is trying to visualize GFP-transfected
>Leishmania, highly motile 10 um flagellated bugs. Does any one have
>experience with things like agarose or something of the kind to
>immobilize the parasites, yet keeping them alive ? Also, what would
>be a good fixative for GFP (as I recall from previous postings,
>there are chemicals that kill GFP fluorescence).
>
>Many thanks,
>
>Renato
>
>Dr. Renato Arruda Mortara
>Disciplina de Parasitologia
>Escola Paulista de Medicina - UNIFESP
>R. Botucatu 862 6o. andar
>04023-062 São Paulo SP
>fone: 55 11 5579-8306
>fax:   55 11 5571-1095
>email: <mailto:[log in to unmask]>[log in to unmask]
>home page: www.ecb.epm.br/~renato

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024       email: [log in to unmask]
___________________________________________________________________________=