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Mark Cannell wrote:

>
> My argument for opening the pinhole a bit when using deconvolution would
> be that as the pinhole is opened sub resolution beads get brighter.

Both the impact of deconvolution on your data and signal strength vs.
pinhole size are very
much object dependent. For point and horizontal line-like objects signal
strength  from the objects themselves
does not increase that much beyond say 1 Airy disk diameter pinhole --
assuming a microscope
performing close to theoretical expectations as Perveen says it is doing.
For other objects, microscopes with lower resolution than expected,
things will work out very
differently.
As George Mcnamara remarked, best is to do a couple of experiments.

Best wishes to all, Hans, a 'Manufactorer'.

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