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Hi
Zucker had a great talk on this in Savannah, MSA meeting.
ALby
On Martedì, ago 10, 2004, at 16:48 Europe/Rome, Locknar, Sarah A wrote:
>
> A standard lamp calibration is necessary if you'd like to record a
> meaningful spectrum of your dye. It allows you to correct for the
> wavelength-dependent transmission of your system. As discussed many
> times in this list, all of the optical components in the system absorb
> light at some wavelengths. A standard lamp allows you to correct for
> these absorptions (instrument response curve). Also, if one of your
> optics begins to fail it should show up in the instrument response
> curve.
> Sarah
>
> -----------------------------------------------------------------------
> ----------------
> Sarah Locknar, Ph.D.
> Director, Neuroscience COBRE Imaging / Physiology Core
> College of Medicine, University of Vermont
> E015 Given Building
> 89 Beaumont Ave.
> Burlington, VT 05405
> 802-656-0413
> 802-656-8704 (fax)
> -----------------------------------------------------------------------
> ----------------
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Guy Cox
> Sent: Monday, August 09, 2004 7:47 PM
> To: [log in to unmask]
> Subject: Re: Spectroscopy Confocal QA
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Sounds interesting - please do send me a copy.
>
> But my question is why is this necessary? You have absolute standards
> built in the in the form of your CW lasers (6 lines on our system) -
> their position shows how accurately your system is calibrated and the
> FWHM shows the resolution. What more do you need?
>
> Guy
>
>
>
> Quoting Robert Zucker <[log in to unmask]>:
>>
>> At the recent microscopy meetings (MM2004) in Savannah a paper by R
>> Zucker and J. Lerner was presented entitled “Confocal Microscopy
>> System
>> Performance: Foundations for Measurements, Quantitation and
>> Spectroscopy
>> â€
>>
>> This Confocal QA spectroscopy test was developed to measure spectral
>> performance and to serve as guidelines for investigators to assess
>> both the performance of their instruments as well as the quality of
>> their data. This spectral characterization test is well suited to all
>> wavelength dispersive CLSM systems including the Leica SP series (SP),
>> the Zeiss LSM510 Meta (Meta) and Olympus FV1000 confocal microscopes.
>> We used an inexpensive, eye-safe, battery operated, multi-ion
>> discharge lamp (MIDL) (LightForm, Inc., Hillsborough NJ) containing
>> mercury ions and inorganic fluorophores as an absolute reference light
>> source because it emits stable, reproducible, peaks between 400 and
>> 650 nm. It is worth applying this test to your systems.
>>
>> An abstract describing this presentation has been published and is
>> available on request.
>>
>> Bob
>>
>>
>> Robert M. Zucker, PhD
>> U.S. Environmental Protection Agency
>> Office of Research and Development
>> National Health and Environmental Effects Research Laboratory
>> Reproductive Toxicology Division, MD 72 Research Triangle Park, North
>> Carolina, 27711
>> Tel: 919-541-1585; fax 919-541-4017
>> e-mail: [log in to unmask]
>
>
> --
> Associate Professor Guy Cox
> Electron Microscope Unit, F09
> University of Sydney NSW 2006
> +61 2 9351 3176
>
>
------------------------------------------------------------------------
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Alberto Diaspro
Department of Physics, University of Genoa
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