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Hi,

In order to know what the appropriate sampling rate/magnification is,
you have to start at least from the Numerical Aperture of the Objective.
  Oversampling a bit (2.3 or 3), but beyond that it doesn't add detail
to the quality of the image.

Magnification as such is only a means to enlarge the image created by
the microsocpe, so we (or a camera) can distinguish the details of the
image created by the microscope.

For more information take a look at:

http://ourworld.compuserve.com/homepages/pvosta/pcrnyq.htm
and
http://www.ph.tn.tudelft.nl/People/young/manuscripts/QM/QM.html

Regards,

Peter

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Peter Van Osta

Director Imaging
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