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Kate-
Somebody sells a chamber into which you can insert your whole
inverted microscope.  This is what we did many years ago in order to
record cell movements in phase microscope mode.  The lens heaters are
a problem because the stage and sample are at different temperatures
than the lens, and it is hard to create a stable gradient of
temperatures.  If this isn't clear, write back or try to find our
paper, Manger and Heckman in the SIVB journal, Tissue Culture Methods.
Carol


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Can anyone comment on their experience with objective heaters? I
>have always heard that the cycles of heating and cooling the
>objectives with objective heaters degrades the lens. Is this true?
>Are some brands better than others? Are there similar  problems that
>arise with chambers like the Solent environmental chamber? What if
>you have some users who want their samples at room temperature, and
>some who want 37°C?
>
>Thanks for your help.
>
>Kate L-P
>
>--------------------------
>Kate Luby-Phelps, Ph.D.
>Director, Live Cell Imaging Core Facility
>Associate Professor, Dept. of Cell Biology
>UT Southwestern Medical Center
>5323  Harry Hines Blvd.
>Dallas, TX 75390-9039
>
>T:  214-648-0429
>F: 214-648-8439
>email:  [log in to unmask]</x-flowed>

--
__
Carol A. Heckman, Ph.D.
Professor of Biological Sciences
Director, Center for Microscopy & Microanalysis
Bowling Green State University, Bowling Green, OH 43403
fax: (419) 372-2024       email: [log in to unmask]
http://www.bgsu.edu/departments/biology/facilities/MnM
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