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Subject:	DRAQ-5 and tissue sections
From:	"Maher, Robert" <[log in to unmask]>
Reply To:	Confocal Microscopy List <[log in to unmask]>
Date:	Wed, 11 May 2005 14:29:38 -0400
Content-Type:	text/plain
Parts/Attachments:	text/plain (22 lines)

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Does anyone have any experience with labeling sections with the nuclear
probe DRAQ-5 (BioStatus Ltd.)?  I'm specifically interested in the mounting
medium chosen.  I labeled tissue sections with DRAQ-5 and mounted the
sections with ProLong Gold (Molecular Probes).  After a day the staining is
significantly decreased.  I've also mounted the sections in water and
glycerin and have had somewhat better success.  Any insight would be
appreciated.

Robert Maher, Scientist
Safety Sciences Michigan-Ann Arbor
Pfizer Global Research and Development
e-mail:  [log in to unmask]




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