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David-

I just tried this on my copy of Leica Confocal Software program and I didn't
have any trouble with it.  I'm using version 2.61, LCS 'lite' which is the
evaluation version. (Our Leica system is on order, but I'm playing with the
demo software to become familiar with the program in advance of the scope's
arrival.)

Here's what I did...

1)Loaded an image stack using the 'Open' Tab at the top.

[Image stack loaded]

2)Click on 'view' arrow near left bottom of the screen.

3) Under the 'View' arrow, I selected 'sect.' tab. The graphic has an image of
a pair of scissors on it.

[Two extra boxes appear-- one below and one to the left of the XY image
representing XZ and YZ views respectively. Initially a pair of dark gray lines
meet at dead center in the XY view. ]

4)Dragged the lines to locate single slices of interest in XZ or YZ views.

5) Right-clicked on the XZ box.  [It became outlined by a dotted white line.]

6) Chose to 'send'.  Here you have an option of sending to 'Printer' or
to 'Experiment'.  If you send to 'experiment' you have the option of making
either a snapshot or a raw data file.  Snapshot seems to generate a TIF file
which I presume would be your desired output for publishing.

J. Scott Gens, PhD
Institute for Biological Complexity
Department of Physics
Indiana University
Bloomington Indiana 47405





Quoting David Knecht <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We are trying to figure out how to do a X-Z (or other cutting planes)
> reslice of a X-Y dataset captured on a Leica SP2.  To be clear, we
> want single slices through in X-Z, not a full volume turned on edge.
> I thought this would be a pretty standard thing to do on any confocal
> software, and someone told me you could do it with Leica software,
> but I can't see any routines for doing it in our system.  Can anyone
> point me in the right direction?  THanks- Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>