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Hi Sarah,
 
According to Warren Zipfel's Ca data (
http://microscopy.bio-rad.com/products/multiphoton/Radiance2100MP/images/mp_
1.gif
<http://microscopy.bio-rad.com/products/multiphoton/Radiance2100MP/images/mp
_1.gif>  (note: log scale, in Gopper-Meyer units) part of
http://microscopy.bio-rad.com/products/multiphoton/Radiance2100MP/mpspectra.
htm
<http://microscopy.bio-rad.com/products/multiphoton/Radiance2100MP/mpspectra
.htm> ), Indo-1 has more than an order of magnitude change at 750 nm, but
has 2 orders of magnitude lower performance than the other dyes listed (many
of which fluoresce with 1p ex only +Ca). At 750 nm, Cacium green (presumably
+Ca) has slightly higher performance than the others. Given the ability to
load cells with dye-AM esters over a wide range of dye concentrations, other
considerations, such as are your detection optics best optimized for one
dye, may come into play. 
 
Best wishes,
 
 
George

-----Original Message-----
From: Locknar, Sarah A [mailto:[log in to unmask]]
Sent: Tuesday, September 27, 2005 8:47 AM
To: [log in to unmask]
Subject: Mp calcium


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Hi- 
I was wondering if there are any thoughts about the best (brightest/biggest
change) calcium indicator to use for MP applications?  We're currently using
fluo-4, so something with similar on/off rates would be ideal- it just seems
that this may not be the best choice for MP.  We're exciting at 750nm.  

Thanks- 
Sarah 

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Sarah Locknar, Ph.D. 
Director, Neuroscience COBRE Imaging / Physiology Core 
College of Medicine, University of Vermont 
E015 Given Building 
89 Beaumont Ave. 
Burlington, VT 05405 
802-656-0413 
802-656-8704 (fax) 
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