Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Henna,

We do a lot of multipoint imaging with oil objectives on a number of 
systems: ZeissLSM510, Leica SP2, DeltaVision (and also on a Zeiss based 
wide-filed system. We have absolutely no problems to image 10-20 (or 
even more locations) as long the sample is not larger then a ~ 1/2 
object slide. We have the cells in LabTek or Ibidi chambers (or in a 
home made holder with a 24mm cover slip as bottom). We have basically no 
problems with the oil (or with temperature fluctuations). I think the 
"secret" is, that (like it was suggested many time already on this list) 
we have large box-type incubator enclosing the X-Y stage and a large 
part of the microscope. We use different type of "boxes" (Solent, Life 
Imaging Services or EMBL) - they all work fine. Another "trick" is to 
check whether your cell-chamber is coupled to your stage in a 
mechanically stable manner.

Cheers    Gabor

-- 
Gabor Csucs PhD
Light Microscopy Centre ETH Zurich
Inst. of Biochemistry, HPM D 8.1
Schafmattstrasse 18
CH-8093 Zurich, Switzerland 

http://www.lmc.ethz.ch
 
Tel:+41-44-633-6221
e-mail: [log in to unmask]