CONFOCALMICROSCOPY Archives

June 2007

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From:
Tamara A Howard <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 13 Jun 2007 15:27:39 -0600
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Search the CONFOCAL archive at
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On Wed, 13 Jun 2007 16:42:56 -0400
  "J.P. Shields" <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hi everyone,
> I have a colleague asking me why their Aspergillus 
>nidulans won't take up
> Hoechst stain very well until they have fixed briefly 
>(1-2 minutes) in
> formaldehyde/buffer.  Anyone else experienced this 
>problem and (more
> importantly) know why it would be like this?  Off the 
>top of my head, I
> suspect the fixation disrupts the membrane potential, 
>allowing particular
> molecules to enter more readily. However, it could have 
>something to do with
> the chitinous cell wall?
> 
> Thanks!
> John
> 
> John Shields
> EM Lab, 151 Barrow Hall
> Univ. of Georgia
> *******
> Always and never are two words you should always 
>remember never to use.
>  - Wendell Johnson

***************************
Tamara Howard
Cell Biology & Physiology
UNM-HSC
Albuquerque, NM
***************************

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