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June 2007

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From:
Tobias Baskin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 14 Jun 2007 14:11:23 -0400
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Pang,
	Yes, very likely. Calcofluor was developed as an additive for 
laundry detergents. It "brightens clothes" because it binds cellulose 
(in cotton anyway) and there is enough uv around to give a little 
boost to the color of the fabric, esp if it is white. I think 
Calcofluor is a specific trade mark and other companies call the same 
(similar?) thing by different names. I expect the Sigma folks can say 
whether Calcofluor is a synonym for their product.

	Tobias

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>I have a bottle from Sigma and it is called Fluorescent Brightener 
>28 (F6259).  Could this be the one Sarah is looking for?
>
>--
>Pang (Wai Pang Chan, [log in to unmask], PAB A087, 206-685-1519)
>The Biology Imaging Facility (http://staff.washington.edu/wpchan/if/)
>
>On Thu, 14 Jun 2007, Tobias Baskin wrote:
>
>>Sarah,
>>	Calcofluor is marketed under various names. One of them is 
>>cellufluor. I think there are others too. I have not tried it on 
>>intact leaves but I bet it would be fine.
>>	Tobias
>>
>>>Hello All,
>>>I have been researching Calcofluor and it seems to be a great dye 
>>>for the imaging of cell walls.  Does anyone have any experience 
>>>using this die on intact leaves?  Any tips?
>>>
>>>Also I am having trouble finding a supplier of this dye.  I have 
>>>not found it in Invitrogen's or Polyscience's catalogs---any hints?
>>>
>>>Thanks,
>>>Sarah Braun, Brock University


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       _      ____          __   ____   
      /  \   /          / \    /   \ \        Tobias I. Baskin
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