CONFOCALMICROSCOPY Archives

June 2007

CONFOCALMICROSCOPY@LISTS.UMN.EDU

Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
From:
Tobias Baskin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 22 Jun 2007 18:01:03 -0400
Content-Type:
text/plain
Parts/Attachments:
text/plain (29 lines)
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Greetings,
	Rosemary White wrote:

>
>And all DNA stains (the ones I've ever used, anyway) also stain the 
>cell walls, of course.
>
  	This is my experience too. But does anyone know why? Is it 
simply the negative charges in phosphates and sugar acids being 
alike? But dyes like propidium idodide (PI) for example are 
supposedly DNA intercalators, and I envisoned this as being steric 
rather than ionic. PI is a great cell wall stain. Anyone know why??

	Thanks,
		Tobias
-- 
       _      ____          __   ____   
      /  \   /          / \    /   \ \        Tobias I. Baskin
     /   /  /          /   \   \      \         Biology Department
    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
   /      /          /       \   \       \        University of Massachusetts
  /      /          /         \   \       \	    Amherst, MA, 01003
/      / ___   /           \   \__/  \ ____
http://www.bio.umass.edu/biology/baskin/
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

ATOM RSS1 RSS2