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Date: | Fri, 29 Jun 2007 19:12:42 -0700 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>I like the Hamamatsu ORCA AG for fixed and live cell fluorescence
>imaging. Very briefly...For live cell fluorescence imaging you
>want a cooled CCD camera that has high quantum efficiency (peak of
>60% or more in the wavelengths you will be imaging) and low read
>noise (8 electrons or less). If you are doing high resolution work,
>you want small pixels (7um or less is small enough for most of us).
>The capacity to do binning is also very useful for live cell
>imaging. I strongly recommend you do some reading as well. Take a
>look at: Spring (2000) BioTechniques Vol 21 (1):76. There are also
>good chapters on cameras in Digital Microscopy, 3rd Ed (Sluder &
>Wolf, eds). Appendix 3 in the 3rd edition of the "Confocal Bible"
>(Handbook of Biological Confocal Microscopy, Pawley, ed) is also a
>great resource. (Jim, haven't you made this available online?)
>
>Best, Jennifer
Hi all,
Yes, it can be found at:
http://www.springer.com/0-387-25921-X
Look under "Additional information" on the right and click the lower
of the 2 articles.
Cheers,
Jim P.
--
****************************************
Prof. James B. Pawley, Phone: 604-822-7801
3D Microscopy of Living Cells: Summer Course CELL: 778-861-2874
"If it isn't diffraction, it is statistics":Microscopist's complaint, Anon.
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