CONFOCALMICROSCOPY Archives

June 2007

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From:
Michael Cammer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 5 Jun 2007 00:06:37 -0400
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Has anybody done this on a consumer or profession DSLR like the Nikon D70
or D80?

I know this is a little off topic, but I'm far more hesitant to try
cleaning these than I've been with TV tube and cooled CCD cameras.  (In
the old days we used freon like it was water and it worked really well!)

-Michael



> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> The First Contact product works wonderfully well on extended surfaces such
> as glass flats, mirrors and prisms.  I have used it on a number of CCD
> windows very successfully.
>
>   You may be so impressed with the results that you are tempted to clean a
> microscope objective  - DON'T.  If it seeps up into the lens assembly
> you risk permanently "locking" the last lens element. The surface,
> however, will be very clean!
>
>   Perhaps it is possible to clean an objective lens, but be sure to speak
> with customer service at Photonic Cleaning first.
>
>   Jeremy
>
> Chris Tully <[log in to unmask]> wrote:
>   Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I have seen a
> product called First Contact made by Photonics Cleaning used by the
> factory reps from Q-Imaging to clean their demo ccd cameras and been
> extremely impressed.  This stuff is very similar to rubber cement.  You
> paint it on to the surface to be cleaned, embed a paper tab in it and
> wait a few minutes.  then peel off the cleaning cement and it will strip
> off finger prints, dust etc.
>
> http://www.photoniccleaning.com/
>
> Chris Tully, M.Sc.
> Applications Engineer
> Vashaw Scientific, Inc.
> 7429 ACC Blvd Suite 101, Raleigh, NC 27617
> Voicemail & Cell: (800) 874-9986 X339
> Fax: (770) 441-7837
> [log in to unmask]
> www.vashaw.com
>
>   On 6/4/07, Armstrong, Brian <[log in to unmask]> wrote:   Search the
> CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I have done this in the past with cotton-tipped applicators and methanol.
> I dislike doing this because it is difficult to get all the dust off. You
> simply need to start in the center of the sensor and in a circular pattern
> move from the center to the edge. You will need to repeat this many times
> using a new applicator each time until the sensor window is clean. Use
> very little methanol on the cotton tip. It seems it is partly by luck when
> you can get it entirely dust free. I have not found canned air to be
> useful for this purpose.
> Good luck,
>
> Brian D Armstrong PhD
> Light Microscopy Core Manager
> Beckman Research Institute
> City of Hope
> 1450 E Duarte Rd
> Duarte, CA 91010
> 626-359-8111 x62872
> http://www.cityofhope.org/SharedResources/LightMicroscopy
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask] ] On
> Behalf Of F Javier Diez Guerra
> Sent: Monday, June 04, 2007 8:45 AM
> To: [log in to unmask]
> Subject: cleaning CCD window
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We have a Coolsnap FX mono behind a Sutter
> lambda-10 emission filter wheel, that is  used for epifluorescence
> microscopy.
>
> From time to time I check illumination flatness,
> alignments, etc. However, when I look at
> transmission samples using brightfield or
> phase-contrast I notice small particles of dust
> located always at same position of the image.
>
> I have examined the microscope and the dust seems
> to be located on the glass in front of the CCD of
> the camera. Careful cleaning with anti-static
> clothe and air-stream only changes the distribution of the dust particles.
>
> After repeating several times the same operation,
> I have realized that the dust comes from small
> particles released in the process of screwing the
> camera into the filter wheel thread.
>
> Has anybody experienced something similar? Any
> recommendation to keep clean the CCD window?
>
> Thanks,
>
>
> F Javier Diez-Guerra, PhD
> Profesor Titular
> Centro de Biologia Molecular Severo Ochoa
> Facultad de Ciencias, Universidad Autónoma
> Ctra Colmenar Viejo Km 15
> Cantoblanco, 28049 Madrid
> SPAIN
>
> phone:  +34 91 4978051
> Fax:      +34 91 4978087
> e-mail: [log in to unmask]
>
>
> "EMF <COH.org>" made the following annotations.
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>
>
>
>
> Jeremy
>
> Tel:  (908) 281-9098
> Cell: (908) 963-4262
> email: [log in to unmask]
> Web:   www.lightforminc.com


_________________________________________
Michael Cammer   http://www.aecom.yu.edu/aif/

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