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That's right, Stephen.

If people are interested in a decent HEPES -buffered solution for all 
sorts of isolated tissue or cell physiology, I can provide it again. 
Otherwise have a look on PubMed for relevant  papers by me (Gibbins IL) 
and or Judy Morris, who actually worked out the optimal solution 
(Morris JL): we always (I think!) give the full recipe.

hope that helps

IAN

On Tuesday, June 5, 2007, at 10:06  AM, Stephen Cody wrote:

> Dear List,
>
>  
>
> Hepes comes up every now and again. I’ve never had a problem with it, 
> but heard all the stories. One thing that has been mentioned is that 
> it may not be the Hepes per se causing the toxicity but rather the 
> lack of bicarbonate ions. I think it was Ian Gibbins who brought that 
> up on this list on18th Jun, 2004.
>
>  



* * * * * * * * * * *
Prof Ian Gibbins
Anatomy & Histology
Flinders University
GPO Box 2100
Adelaide SA 5001
AUSTRALIA

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http://som.flinders.edu.au/FUSA/Anatomy/
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