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Hello,

Sorry to ask such a low tech question, but has anyone tried or have opinions
about blue LED adapted scopes for visualizing EGFP?  I have gotten fairly
good results from large tissue blocks expressing EGFP using a strong blue
LED flashlight, but I don't know how feasible it is for sections.  Here are
two links for do-it-yourself guides.

http://www.plantsci.cam.ac.uk/Haseloff/imaging/cheaposcope/cheaposcope.htm

http://130.15.90.245/gfp_stereoscope.htm

In particular I am thinking about adapting a dissecting microscope for very
gross visualization of EGFP in brain sections.  The sections will probably
be from 50 microns to 1 mm.  Does anyone have experience with this or
suggestions?  

Thanks,

Caroline