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I am also interested in a light source for student scopes, but at  
$3000-4000, these LED light sources are not exactly cheap.  A 50W  
mercury illuminator is about half that price.  It makes sense to not  
have the expense or hassle of mercury lamps in a student lab, but it  
sure would be nice to have a cost effective solution.  Dave

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)


On Jul 20, 2007, at 4:00 AM, Rietdorf, Jens wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Caroline,
>
> We use the 465nm LED Array Modules of the precisExcite product from
> CoolLED Ltd in combination with a regular EGFP filterset from Chroma
> with good success. They state their 465nm LAM offers greater power  
> than
> your 100W mercury bulb, which is well possible, because the Hg arc has
> no peak around this region.
>
> No commercial interest.
>
> regards, jens
>
> ---
> Dr. Jens Rietdorf
> Head Microscopy
> Novartis Research Foundation
> Friedrich-Miescher-Institute, wro1066.2.32
> Maulbeerstr.66, CH-4058 Basel, Switzerland
> phone +41(61)69-75172 mobil +41 798284737
> Email:[log in to unmask]
>
> -----Original Message-----
> From: Confocal Microscopy List  
> [mailto:[log in to unmask]] On
> Behalf Of Caroline Bass
> Sent: Freitag, 20. Juli 2007 06:03
> To: [log in to unmask]
> Subject: Student EGFP scope
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello,
>
> Sorry to ask such a low tech question, but has anyone tried or have
> opinions about blue LED adapted scopes for visualizing EGFP?  I have
> gotten fairly good results from large tissue blocks expressing EGFP
> using a strong blue LED flashlight, but I don't know how feasible  
> it is
> for sections.  Here are two links for do-it-yourself guides.
>
> http://www.plantsci.cam.ac.uk/Haseloff/imaging/cheaposcope/ 
> cheaposcope.h
> tm
>
> http://130.15.90.245/gfp_stereoscope.htm
>
> In particular I am thinking about adapting a dissecting microscope for
> very gross visualization of EGFP in brain sections.  The sections will
> probably be from 50 microns to 1 mm.  Does anyone have experience with
> this or suggestions?
>
> Thanks,
>
> Caroline