CONFOCALMICROSCOPY Archives

July 2007

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Re: Single-molecule imaging with an Olympus Fluoview confocal microscope?
From:
Ian Dobbie <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 31 Jul 2007 13:55:23 +0100
Content-Type:
text/plain
Parts/Attachments:
text/plain (21 lines) 
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

David Knecht-charter <[log in to unmask]> writes:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Given Martin's comment, what is a good simple and conclusive test for
> determining sensitivity in the 1-10 fluorophore/molecule range if
> testing cameras/systems?  Dave

In a TIRF system you should be able to get stepwise photobleaching to
see how many fluorophores you have:
Nature 443, 355-358 (2006)
Stoichiometry and turnover in single, functioning membrane protein complexes
Mark C. Leake, Jennifer H. Chandler, George H. Wadhams, Fan Bai1, Richard M. Berry  and  Judith P. Armitage


Ian

ATOM RSS1 RSS2