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November 2007

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From:
Mark Cannell <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 12 Nov 2007 09:09:32 +1300
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George McNamara wrote:
>
>
> STED Question: what happens to the fluorophore's excited energy when 
> depletion occurs? Does the molecule return to the ground state (a) 
> without emitting a photon or (b) emits a photon of the same wavelength 
> (and direction? as in a laser) as the depletion photon?
Hi George

As I understand it, in Stimulated Emission, the molecule emits a photon 
similar just like as occurs in a laser. This is possible because the 
emission spectrum is broad (lots of vibrational levels possible).

Cheers Mark Cannell

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