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Dear Dmitry,

you won't be able to see the lamp filaments after passing through the 
fiber. So yes, if you want to align the lamp in a proper way, you have 
to remove the fiber from the lamphouse and align it at the wall (or an 
another stand).

I have to say that I so far did it also based on highest throughput, but 
I know about the security issues and will (hopefully) do it better soon. 
The fiber is anyway killing a lot of light, so on a longterm view it 
might be better to go for other solutions, i.e. LED sources or stronger 
Metal Halide lamps with better fibers.

cheers,
Michael


Dmitry Sokolov wrote:
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear All,
> 
> May I ask you for your suggestion on proper procedure of mercury arc 
> bulb alignment of fiber-coupled 106 z lamp housing. I was recommended to 
> align it by maximum of intensity of light passing through the objective 
> of (Leica DM6000B) microscope . The intensity and uniformity is 
> controlled looking on a sheet of paper placed under turret with 
> objective removed.
> 
> The corresponding "Leica DM6000B DM6000M Operating manual" has a warning 
> of lamp explosion by overheating if "The bright tips of the arcs, the 
> focal sports..." projected onto each other (p.55) or contact elements of 
> lamp. The alignment procedure is based on viewing the arcs through a 
> special window of the microscope. In my case I see no images of arcs but 
> a number of rings originated probably from the fiber ends.
> 
> Should I better remove the fiber coupler and align the lamp looking on 
> it's image on the wall?
> 
> I would highly appreciate it if you could share your experience.
> 
> Cheers,
> Dmitry
> 
> ___________________________________________
> Dr. Dmitry Sokolov,
> Manager of Confocal Microscopy Unit
> Institute of Molecular Biosciences
> Riddet Road, Massey University
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