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Dear Glen,
The FWHM of the 525nm LED is about 50nm, its working for Alexa546,
Alexa568, Cy3, Cy3.5 etc.
We don't control it from the LSM software.
regards, jens
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Glen MacDonald
Sent: Dienstag, 6. November 2007 17:59
To: [log in to unmask]
Subject: Re: Non-arc source for IX-81
Search the CONFOCAL archive at
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Dear Jens,
How well do red fluorescing dyes respond to the 525, or do you routinely
use those wavelengths?
Did you use their API to incorporate wavelength switching with the LSM
software?
Regards,
Glen
On Nov 6, 2007, at 7:09 AM, Rietdorf, Jens wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Glen,
>
> We have replaced the arc-lamps on 2 of our facilities Zeiss confocals
> now 6 month ago by the CoolLED Ltd solution. We use 400nm, 465nm,
> and
> 525nm, and it works well (no complaints from users so far). We
> modified the filtercubes to have max ex efficiency. I understood
> CoolLEDs are also sold with Olympus adapters.
>
> cheers, jens
>
> ---
> Dr. Jens Rietdorf
> Head Microscopy
> Novartis Research Foundation
> Friedrich-Miescher-Institute, wro1066.2.32 Maulbeerstr.66, CH-4058
> Basel, Switzerland phone +41(61)69-75172 mobil +41 798284737
> Email:rietdorf(at)fmi.ch
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Glen MacDonald
> Sent: Montag, 5. November 2007 20:02
> To: [log in to unmask]
> Subject: Non-arc source for IX-81
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Has anyone replaced the mercury illuminator on an Olympus IX-81 with a
> non-arc source such as metal-halogen or LED? I'm looking for
> experiences regarding reliability, operational cost and performance on
> a laser scanning confocal installation.
>
> Off-line commercial responses are welcome.
> Thanks,
> Glen
>
>
>
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center Box 357923 University
> of Washington Seattle, WA 98195-7923 USA
> (206) 616-4156
> [log in to unmask]
>
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