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We and many others have successfully used the combination of
Cerulean(CFP)/Venus(YFP)/mCherry when we have needed three genetically
encoded fluorescent proteins.  

Anyone care to comment on successes using four?

--
Samuel A. Connell                                        
Director of Light Microscopy
Cell & Tissue Imaging Center
St. Jude Children's Research Hospital
332 North Lauderdale St., E7061
Memphis, TN 38105-2794
(901) 495-2536 
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Xinyu Zhao
Sent: Thursday, November 08, 2007 9:07 AM
To: [log in to unmask]
Subject: combination of 3 fluorescence protein reporters. .

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Dear listers,

One of our users wanted to image CFP and YFP labeled cells and would
like to 
add a third fluorecence protein reporter later. I was wondering if
anybody 
could make a recomendation on this third one. 

Anybody has ever used a  CFP/YFP/mCherry combination ?

Thank you very much.


Xinyu Zhao
Biomedical Imaging Core Lab
School of Medicine
University of Pennsylvania
Tel:  215-898-6730