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Hi, Alice,

We did not use the Zeiss camera. However, we had a similar need long time
ago as some one tried to correlate histology with immunofluorescence. Here
was how we got by (without a camera): use your red, green and blue laser
lines and set up a sequential scanning configuration with your transmitted
detector. You can get a pseudo-color image which might need some tweaking
(mostly gamma) by the end. Most importantly, you can just use the tilscan
function of your LSM and get your color tiled images. If you calibrate the
stage, you can get a reasonable tiled image out of it. But I think Zeiss
software limits the final frame size (in term of number of pixels).

It has been a long time we did this and I might forget some details. Initial
setting up (gain/offset of the detector) was a bit pain in term of getting
correct colors. But once set, you can reuse it and tweak the final image in
photoshop. Additionally, If I recall correctly, one critical thing is to
remove DIC optics from the optical path or you might get a gradient in your
tiled image.

Good luck,

Xuejun


Xue-jun Sun, Ph.D.
Dept. Exp. Oncology
Cross Cancer Institute
11560 University Ave,
Edmonton Alberta T6G 1Z2
Canada
Phone: (780) 432-8898
Fax:     (780) 432-8425

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Alice L. Givan
Sent: Wednesday, November 07, 2007 3:26 PM
To: [log in to unmask]
Subject: CCD camera

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We would like to add a color CCD camera to our Zeiss LSM-510 META confocal
to capture color tiled wide field images.  We wondered if other users have
already done this?  What cameras did you add and how well does the LSM 510
software control the image acquisition with the camera you are using?  We
are particularly interested in tiled images.

We have a PixeLINK model PL-A662 color CCD camera on a separate stereo
scope.  We are told that this camera would not work with the LSM-510.  Does
anyone have any contrary information about this?

Thanks for your  help.

Alice

Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, NH 03756 USA
tel 603-650-7661
fax 603-650-6130
[log in to unmask]
www.dartmouth.edu/~celllab


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