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Dear Farid and Christophe,

I am not sure if this is useful for you, but have a look at the Ibidi 
grid dish:

http://ibidi.com/products/dish_grid.html

This might help to locate the sample over the whole process of culturing 
and acquisition. Maybe it's an option to home-made solutions.

I am pretty confident that some time ago, I also saw prepared coverslips 
like this, but don't remember where.

Michael


Christophe Leterrier wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> I'd be very interested to hear answers about the fiducial mark issue too...
> 
> Christophe Leterrier
> 
> On Feb 20, 2008 2:04 AM, Farid Jalali <[log in to unmask]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello Group,
>> 1) Can anyone offer a suggestion for free/share-ware to drive simple image
>> acquisition using a Sony XCD-X700 IEEE 1394 camera? Its attached to our
>> Axiovert 100 and we use it for basic brightfield/ phase contrast
>> fluorescence image acquisition.
>>  2) Does anyone have a suggestion for marking #1.5 coverslips more
>> permanently? I have tried a diamond tipped pen but the score was too deep
>> and media made its way through. We have tried nail polish, but it seems to
>> eventually wash away as does permanent marker. I am generating
>> laser-mediated tracks of DNA damage and want to be able to orient my
>> coverslips after I have fixed and stained the samples.
>>
>> As always, any help or suggestions would be greatly appreciated.
>> Cheers
>> Farid
>> --
>> Farid Jalali MSc
>> Senior Research Technician/ Lab Manager
>> Dr. Robert Bristow Lab
>> Applied Molecular Oncology
>> Princess Margaret Hospital
>>  Toronto, Canada
>> 416-946-4501 X4351 (Princess Margaret Hospital)
>> 416-581-7754 STTARR at MaRS Building
>> 416-581-7791 STTARR Microscopy Suite