Hi 

I'm new to this forum. I have tried searching this forum but I can't find a
topic. I apologize if I missed it. 

I'm a PHD student I'm relatively new to confocal imaging I am trying to
image sub resolution beads to measure the point spread function. 

I am using a bio rad system I use a 60X water immersion lens I set the
system to 1024 X 1024 at 16 bit resolution. Kalman sampling was set to 4 and
the scanning head was as slow as possible and the laser power was as low as
possible to measure the beads.

I have found that this is the perfect recipe for measuring photo bleaching! 

I tried looking around for some sort of protocol on imaging sub resolution
beads but I was unable to find anything. Also as I understand it there will
always be some element of photo bleaching? Is it easy to compensate for if I
can calculate how much beaching as occurred. 

Similarly if anybody as any tips on creating the slides with the beads I
would be grateful mine are quite messy. 

Many thanks 

Stuart McIntyre 


-- 
View this message in context: http://n2.nabble.com/Protocol-for-imaging-micro-beads--tp1571444p1571444.html
Sent from the Confocal Microscopy List mailing list archive at Nabble.com.