Hi Claire,
            We have had some good results using LavaCell from Fluorotechnics
 
http://www.fluorotechnics.com/
 
It works well, sometimes not that bright unfortunately, but is very persistent. Cells still carry stain after 48 hours, and it is still only at the membrane.
 
 
Cheers
 
 
Cam
 
 
 
Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
 
Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104
 
http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
 

________________________________

From: Confocal Microscopy List on behalf of Jamie Barber
Sent: Thu 12/03/2009 12:24 AM
To: [log in to unmask]
Subject: Re: Membrane Staining



Hi Claire, just a thought, can your researcher grow the polarized cells in a Transwell or similar insert?  Any dye would then have access to the basal surfaces...

 

Jamie Barber
AHRC Cell Imaging Facility
Dept. of Infectious Diseases
Univ. of Georgia, College of Veterinary Medicine
111 Carlton St.  Bldg 1077
Athens GA 30602
Office: (706) 542-4092
Laboratory: (706) 542-9862
[log in to unmask]

www.vet.uga.edu/ID/tripp/personnel/Jamie

 

 

 

From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Claire Brown, Dr.
Sent: Wednesday, March 11, 2009 9:13 AM
To: [log in to unmask]
Subject: Membrane Staining

 

I have a user who is working with polarized epithelial cells and wants to label the basal-lateral membrane with dye. She tried FM4-64 but it is not penetrating the tight junctions so it is only labeling the apical surface. She wants to do this on live cells and would prefer a dye to a fluorescent protein. Any suggestions would be helpful.

 

Sincerely,

 

Claire

 

Claire M. Brown, PhD

Life Sciences Complex Imaging Facility Director

McGill University Department of Biochemistry

Montreal, Quebec, H3G 0B1

http://www.lifesciencescomplex.mcgill.ca/imaging <http://www.lifesciencescomplex.mcgill.ca/imaging>