CONFOCALMICROSCOPY Archives

April 2010

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Re: Non Enzymatic Cell Harvesting Buffer
From:
F Javier Díez Guerra <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 26 Apr 2010 19:11:50 +0200
Content-Type:
text/plain
Parts/Attachments:
text/plain (19 lines) 
El 26/04/2010 18:25, B. Prabhakar Pandian escribió:
> Hello,
>          We are trying to harvest adherent cells following activation 
> with IL-1 for certain receptor upregulation. However, we  feel that 
> the trypsin-EDTA mixture used for harvesting is cleaving the receptors.
> Can somebody suggest a proven non-enzymatic cell harvesting solution.
>
>
> Thanks,
>
> -Prabhakar

Hi,

Using cold PBS-EDTA (no trypsin), adherent cells detach although more 
slowly.

Javier

ATOM RSS1 RSS2