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Hi Hana,

Try and place a bubble trap into your system and make sure it is placed
downstream of the pump, but before entry to the chamber. This should fix
that problem. Alternatively you could use a syringe pump instead of a
peristaltic pump which gives a much smoother flow. 
As for the other problem, depending on the design you could be creating
"pockets" where liquids are not being exchanged as readily as in other
areas which will be a real nightmare when it comes to the analysis of
your images. Make sure there are plenty of entry points within your
chamber to allow the even passage of liquids through your system also.
This will ensure you achieve laminar flow. To know whether you are
achieving laminar flow you can calculate the Reynolds number for the
system. If it is under 2300, you have laminar flow. 

Kind regards,
Deanne.

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Hana Uhlirova
Sent: Wednesday, 27 October 2010 12:43 AM
To: [log in to unmask]
Subject: perfusion system

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Dear list,

Has anyone experiences with perfusion system for live cell imaging?
Particularly I need to rinse with two liquids of precise concentration.
We
have a home-made closed system and have problems with air bubbles and
mixing
of those liquids by loss of the concentration accuracy. Any tips how to
figure this out?

Thank you all.

Hana

Hana Uhlirova PhD
Laboratory of Optical Microscopy
Institute of Physical Engineering
Faculty of Mechanical Engineering
Brno University of Technology
Czech Republic