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The Dermainspect system in Europe is basically a 2 photon microscope with
special gear to allow it to image skin on a person. The goal was to check
moles/freckles for signs of cancer.
http://www.jenlab.de/DermaInspect-R.29.0.html
(no commercial relationship)
Craig
> (pps: now that I am thinking about it there are people using laser scanning
> techniques for non-invasive imaging under biological surfaces (ie. Skin) but
> I am not if the short and long term consequences have been fully
> investigated!)
>
>
>
> Eric Scarfone, PhD, CNRS,
> Center for Hearing and communication Research
> Department of Clinical Neuroscience
> Karolinska Institutet
>
> Postal Address:
> CFH, M1:02
> Karolinska Hospital,
> SE-171 76 Stockholm, Sweden
>
> Work: +46 (0)8-517 79343,
> Cell: +46 (0)70 888 2352
> Fax: +46 (0)8-301876
>
> email: [log in to unmask]
> http://www.ki.se/cfh/
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of JOEL B. SHEFFIELD
> Sent: 16 October 2010 19:00
> To: [log in to unmask]
> Subject: Re: Cheap or free sources of cells
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I suppose you can expand this demo by taking a later swab and seeing how
> long it takes to replace the dead with the living.
>
> Joel
>
>
> On Thu, Oct 14, 2010 at 4:25 PM, Ignatius, Mike
> <[log in to unmask]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > The nice thing about cheek swab is that you get lots of bacteria as
> > well, which can be used for quick demo.
> >
> > Make the cheek swab prep, then image live vs. dead bacteria with our
> > fluorescent LIVE/DEAD(r) BacLight(tm) Bacterial Viability Kit L7012.
> >
> > Then gargle with either alcohol based or cetylpyridinium chloride
> > (CPC) based mouthwash. Scrap some more cheek cells and see the
> > dramatic increase in dead (red) bacteria.
> >
> > Mike Ignatius
> >
> > Molecular Probes/Life Technologies
> >
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[log in to unmask]]
> > On Behalf Of Philip Oshel
> > Sent: Thursday, October 14, 2010 9:33 AM
> > To: [log in to unmask]
> > Subject: Re: Cheap or free sources of cells
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > How about the old classroom standby: epithelial cells from cheek swabs?
> > Phil
> >
> > >*****
> > >To join, leave or search the confocal microscopy listserv, go to:
> > >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >*****
> > >
> > >Yes, the onion! I had forgotten about that one. And this preparation
> > >by Pawley is a good one since one of the dyes I wanted to test is DiR
> > >(instead of DiO).
> > >
> > >Any more ideas out there? Plant, yeast, and fungi cells are good, but
> > >are there any more animal cell sources?
> > >
> > >Thanks for all replies so far.
> > >
> > >John Oreopoulos
> > >
> > >
> > >
> > >On 2010-10-14, at 11:16 AM, Baggi Somasundaram wrote:
> > >
> > >> *****
> > >> To join, leave or search the confocal microscopy listserv, go to:
> > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >> *****
> > >>
> > >> Hi John
> > >> The use of onion cells for live cell confocal demonstrations
> > >>always works well.
> > >> Link
> > >>
> > >>
> > http://books.google.com/books?id=E2maxdEXFNoC&pg=PA390&lpg=PA390&dq=on
> > ion+cells+for+confocal&source=bl&ots=bgheiJGfN2&sig=64Yas9eXWgEECKbjhC
> > Nafsnx4FA&hl=en&ei=DR63TM7xL4rEsAO9xeC5CQ&sa=X&oi=book_result&ct=resul
> > t&resnum=1&sqi=2&ved=0CBcQ6AEwAA#v=onepage&q=onion%20cells%20for%20con
> > focal&f=false
> > >>
> > >> Baggi Somasundaram
> > >> BioImaging Solutions
> > >> [log in to unmask]
> > >>
> > >> -----Original Message-----
> > >> From: Confocal Microscopy List
> > >>[mailto:[log in to unmask]] On Behalf Of John
> > >>Oreopoulos
> > >> Sent: Thursday, October 14, 2010 7:04 AM
> > >> To: [log in to unmask]
> > >> Subject: Cheap or free sources of cells
> > >>
> > >> *****
> > >> To join, leave or search the confocal microscopy listserv, go to:
> > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >> *****
> > >>
> > >> Dear confocal listserver,
> > >>
> > >> I'm looking for cheap or free sources of cells that can be used
> > >>for testing various fluorescent stains and/or microscopy
> > >>instrumentation. I've had good success in the past with blood cells
> > >>pricked from my finger with a finger lance and cheek cells scraped
> > >>from my mouth. I'm wondering if any of you out there know of any
> > >>other sources of cells for this same kind of purpose. Maybe urine
> > >>samples? Anything from nature/outdoors (besides pond water)?
> > >>Anything from the supermarket?
> > >>
> > >> Looking forward to all your creative responses!
> > >>
> > >> John Oreopoulos
> >
> > --
> > Philip Oshel
> > Microscopy Facility Supervisor
> > Biology Department
> > 024C Brooks Hall
> > Central Michigan University
> > Mt. Pleasant, MI 48859
> > (989) 774-3576
> >
>
>
>
> --
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [log in to unmask]
> URL: http://astro.temple.edu/~jbs <http://astro.temple.edu/%7Ejbs>
>
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