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>is there any numbers? Opinions I already have.
at 800 nm, 2.5% laser power setting (AOM transmission)
25x: 10.4 mW, 20x: 7.9 mW
at 950 nm:
25x: 3.2 mW, 20x: 2.85
at 1020 mW 6%
25x: 2.61, 20x 2.39 mW
these were measured on the Olympus system which fills the back aperture
of the 25x objective, but underfills the 20x objective, when i set the beam expander to get the same fill factor for the 25x as for the 20x, i measure
11.8 mW (800nm), 3.5mW (950), 2.91 mW (1020)
so i conclude about 20-50% more transmission in the IR for the 25x
These values were measured in air, so there is some total reflection expected for the 25x NA1.05 objective. The measured power levels also depend on
the output characteristics of the laser. Furthermore there are some uncertainties depending on the type of power meter used, i think this was done with
a photodiode based sensor (was a while ago).
best wishes
Andreas
On Oct 19, 2010, at 10:02 AM, Andreas Bruckbauer wrote:
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>
>
>
>> I was told recently that the 20x from olympus has better response in
>> red/infrared range than de 25x
>
> the 25x has a higher transmission in the red/IR. To achieve the same laser
power out of the objective e.g you don't want to kill your tissue, you need to
attenuate more. If you are limited in laser power, e.g. at he fringes of the
spectrum and for deep imaging you get more through with the 25x. That is waht is
was designed for.
>
> best wishes
>
> Andreas
>
>
>
>
>
>
> -----Original Message-----
> From: Nuno Moreno <[log in to unmask]>
> To: [log in to unmask]
> Sent: Tue, 19 Oct 2010 9:31
> Subject: Re: 20X objective for 2P work
>
>
> *****
>
> To join, leave or search the confocal microscopy listserv, go to:
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> *****
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>
>
> Hi,
>
>
>
> We use the 20x and its really a workhorse!
>
>
>
> I was told recently that the 20x from olympus has better response in
>
> red/infrared range than de 25x despite of the lower NA. Can anyone confirm
this?
>
>
>
> All the best,
>
> Nuno Moreno, PhD
>
> Head of Equipment Management
>
> Instituto Gulbenkian de Ciência
>
> Fundação Calouste Gulbenkian
>
> Tel . +351 4464538
>
> Fax. +351 4407970
>
>
>
>
>
>
>
>
>
>
>
> On Oct 19, 2010, at 5:33 AM, Adrian Smith wrote:
>
>
>
>> *****
>
>> To join, leave or search the confocal microscopy listserv, go to:
>
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>> *****
>
>>
>
>> Hi all,
>
>>
>
>> Nikon also has a new 25x objective for multiphoton work - specifications look
>
> very similar to the Olympus and it available without buying a complete
>
> system... however I've not had any feedback from actual users yet.
>
>>
>
>> I'm hoping to be able to test one on our LaVision BioTec TriMScopes at some
>
> stage but haven't had any success getting that organised yet.
>
>>
>
>> We've had good results with the Olympus 20x NA 0.95 (standard and special IR
>
> versions).
>
>>
>
>> Regards,
>
>>
>
>> Adrian Smith
>
>> Centenary Institute, Sydney, Australia
>
>>
>
>> On 19/10/2010, at 10:16 AM, Cameron Nowell wrote:
>
>>
>
>>> *****
>
>>> To join, leave or search the confocal microscopy listserv, go to:
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>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>>> *****
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>>>
>
>>> Hi List,
>
>>>
>
>>> Unfortunately the Olympus 25x is only sold as part of a complete system
>
>>> (we have one and it has been great). But the older 20x XLumPlanFL works
>
>>> pretty well too for visible and IR wavelengths and you should be able to
>
>>> buy one of those separately.
>
>>>
>
>>>
>
>>> Cheers
>
>>>
>
>>> Cam
>
>>>
>
>>>
>
>>> Cameron J. Nowell
>
>>> Microscopy Manager
>
>>> Centre for Advanced Microscopy
>
>>> Ludwig Institute for Cancer Research
>
>>> PO Box 2008
>
>>> Royal Melbourne Hospital
>
>>> Victoria, 3050
>
>>> AUSTRALIA
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>>> Office: +61 3 9341 3155
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>>>
>
>>>
>
>>> -----Original Message-----
>
>>> From: Confocal Microscopy List [mailto:[log in to unmask]]
>
>>> On Behalf Of Patty Jansma
>
>>> Sent: Tuesday, 19 October 2010 6:07 AM
>
>>> To: [log in to unmask]
>
>>> Subject: Re: 20X objective for 2P work
>
>>>
>
>>> *****
>
>>> To join, leave or search the confocal microscopy listserv, go to:
>
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>>> *****
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>>>
>
>>> http://www.olympusamerica.com/seg_section/seg_pressDetails.asp?pressNo=6
>
>>> 46
>
>>>
>
>>> I think it may only be sold with the Flo-view1000MPE but does what you
>
>>> want.
>
>>>
>
>>> Patty
>
>>>
>
>>> On 10/18/2010 11:20 AM, Carl Boswell wrote:
>
>>>> *****
>
>>>> To join, leave or search the confocal microscopy listserv, go to:
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>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>>>> *****
>
>>>>
>
>>>> Dear listers,
>
>>>> We're currently on the search for a high NA water immersion 20x
>
>>>> objective to use for intravital 2P imaging. As such, we need
>
>>>> something that does it all, short wavelength transmission for SHG and
>
>>>> long wavelength transmission for our Ti:Saph excitation source. Any
>
>>>> thoughts on what folks use/have seen/would like to have/have heard
>
>>>> about would be appreciated.
>
>>>>
>
>>>> Thanks,
>
>>>> c
>
>>>>
>
>>>> Carl A. Boswell, Ph.D.
>
>>>> Molecular and Cellular Biology
>
>>>> University of Arizona
>
>>>> 520-954-7053
>
>>>> FAX 520-621-3709
>
>>>
>
>>>
>
>>> This communication is intended only for the named recipient and may contain
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>>> The views expressed in this communication are those of the sender and do not
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