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Hello, 

I'm hoping to investigate the regenerative time-course of descending neurons in 
the zebrafish following laser axotomy. This involves the creation of a trangenic 
line of zebrafish which express a fluorescent indicator in a specific cell subset. 
Since the laser can only penetrate so far without baking the surrounding tissue, 
does anyone have a suggestion as to which cells might be easiest to cut and 
visualize? Also, has anyone played around with the wavelength and power 
settings needed to selectively disrupt tissue?

Thank you, 

DRoumis