Thanks to all the replies and suggestions.
I am more in
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Good day:
Thanks to all the replies and suggestions.
I am more interested in knowing the abilities of widefield (low cost)
hyperspectral imaging systems, such as the Pariss one mentioned by Michael
or other such systems in resolving such signals. I think there are a few
commercial systems out there?
Thanks again,
GK
________________________________
From: Michael Weber <[log in to unmask]>
To: [log in to unmask]
Sent: Fri, December 3, 2010 5:29:49 AM
Subject: Re: Hyperspectral imaging systems for GFP YFP
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Dear all,
from Robert Zucker's papers about confocal performance I recall the "Pariss
Hyperspectral Imaging" system. Essentially a wide-field system equipped with a
prism and a detection CCD, which allows to "slit-scan" the field of view and
obtain a spectral image. No idea if this is still available, or how it performs
apart from the data you can find in Robert's papers.
http://www.pariss-hyperspectral-imaging.com/
No commercial interest.
Michael
On Dec 3, 2010, at 8:10 AM, Andreas Bruckbauer wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
>
> Would it be possible to have a widefield hyperspectral microscope? For fast
>aquisition you would have to split the fluorescence somhow into different
>channels and this is usually limited to a low number of spectral channels, like
>four. In principle you could couple a spectrometer to the microscope and measure
>one wavelength at a time but this would be slow. The confocal option seems to be
>more apealing, is the Nikon C1si a point or slit scanning system? M. Sinclair et
>al. Applied Optics (2006) 45 6283 have build a slit scanning hyperspectral
>confocal which would be a good compromise. D. Lidke has used this system to
>separate 5 different quantum dots with a time resolution of 4s/frame (Immunity
>(2009) 31 469). The only system I know which is widefield would be the
>imagestream imaging flow cytometer https://www.amnis.com/ which offers 12
>simultaenous channels but in this case it is the cells which are "scanned" or
>flowing through the image instead of scanning the laser. Does anyone know of
>other options?
>
> best wishes
>
> Andreas
>
>
>
>
>
>
> -----Original Message-----
> From: Daniel Gitler <[log in to unmask]>
> To: [log in to unmask]
> Sent: Fri, 3 Dec 2010 6:28
> Subject: Re: Hyperspectral imaging systems for GFP YFP
>
>
> *****
>
> To join, leave or search the confocal microscopy listserv, go to:
>
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>
> *****
>
>
>
> Again, Nikon C1Si. Seems to be a prevalent answer.
>
> Daniel
>
>
>
> ----- Original Message -----
>
> From: Paul Rigby <[log in to unmask]>
>
> Date: Friday, December 3, 2010 2:19
>
> Subject: Re: Hyperspectral imaging systems for GFP YFP
>
> To: [log in to unmask]
>
>
>
>> *****
>
>> To join, leave or search the confocal microscopy listserv, go to:
>
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>
>> *****
>
>>
>
>> We've also done it in real time with the Nikon A1Si, but never
>
>> widefield.
>
>> Paul Rigby
>
>>
>
>> -----Original Message-----
>
>> From: Confocal Microscopy List
>
>> [mailto:[log in to unmask]] On Behalf Of Craig Brideau
>
>> Sent: Friday, 3 December 2010 2:58 AM
>
>> To: [log in to unmask]
>
>> Subject: Re: Hyperspectral imaging systems for GFP YFP
>
>>
>
>> *****
>
>> To join, leave or search the confocal microscopy listserv, go to:
>
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>
>> *****
>
>>
>
>> We've done it with a laser scanning system (Nikon C1Si) but not
>
>> widefield.
>
>> Craig
>
>>
>
>>
>
>> On Thu, Dec 2, 2010 at 11:30 AM, geekay b <[log in to unmask]> wrote:
>
>>
>
>>> Not a confocal-related question, but I will appreciate if an
>
>>> *****
>
>>> To join, leave or search the confocal microscopy listserv, go to:
>
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>
>>> *****
>
>>>
>
>>> Good day:
>
>>>
>
>>> Not a confocal-related question, but I will appreciate if
>
>> anyone has
>
>>> experience
>
>>> of using Widefield Hyperspectral Imaging Systems for
>
>> separating GFP and YFP
>
>>> signals or autofluorescence in biological samples.
>
>>>
>
>>>
>
>>> Thank you.
>
>>>
>
>>> Sincerely
>
>>> GK
>
>>>
>
>>>
>
>>>
>
>>>
>
>>> ________________________________
>
>>> ,
>
>>>
>
>>>
>
>>>
>
>>
>
>
>
> Daniel Gitler, Ph.D.
>
> Department of Physiology and Neurobiology
>
> Faculty of Health Sciences
>
> Ben Gurion University of the Negev
>
> Beer-Sheva 84105
>
> Israel
>
>
>
> Tel: +972-8-6477345
>
> Cell: +972-54-2110100
>
> Fax: + 972-8-6477628
>
> http://web2.bgu.ac.il/physiology/faculty-members/daniel-gitler/
>
|
