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Dear Amol,
you will rarely be able to affect the projector used for your presentation
or plug your own in, so you better adjust the images accordingly. There are
some simple but bullet-proof solutions to the problem.
First, projectors and colour profiles are produced for hollywood movies,
not scientific presentations, so the internal colour profiles are sigmoid,
not linear, to give 'vibrant colours', but this eliminates the fine, dim
structures typically essential for microscopy results. Also don't forget
that not only our eyes will always be more sensitive to green than red and
blue, but 10% of men can't distinguish red and green anyway. So convert your
images to greyscale, that helps massively - only use colour images for
overlays to show relative localisations. Best use black on white (i.e. black
structures on a white background - inverting the fluorescent images), that
also solves the problem with the room illumination (and people falling
asleep in lunch time seminars).
You also need to be aware that projectors never use the extremes of the
8-bit depth, values close to '0' or '255' won't be visible (usually at least
20-30 values either side). That's why Photoshop offers the OUTPUT LEVELS
adjustment, use it to 'shrink' the histogram to the range supported by the
projector and room illumination (or use reduce it by ~30 either side, if
unknown).
One option is also intensity-independent pseudo-3D presentation (e.g. like
the '2.5D' option in the Zeiss confocal software), which shows intensities
as peak heights.
I hope this helps,
Martin
######################################
Martin Spitaler, PhD
FILM - Facility for Imaging by Light Microscopy
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK
Tel. +44-(0)20-759-42023
E-mail [log in to unmask]
Website: http://imperial.ac.uk/imagingfacility
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