*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Leica has just announced that they now have a coverslip corrected 40x water immersion objective.
15506341 HCX PL APO 40x/1.10 W CORR CS
HCX PL APO 40x/1.10 water immersion objective with correction collar optimized for
confocal scanning applications. High transmission from 360-1100 nm, free working
distance: 0.63 mm. For 405 nm excitation correction lens 155063414 has to be used.
Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
[log in to unmask]
http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Robert J. Palmer Jr.
Sent: Wednesday, March 09, 2011 7:42 AM
To: [log in to unmask]
Subject: Re: 40x water immersion objective on Leica miscroscope
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
The Leica global website currently lists 10x, 20x, 40x and 63 x (the
latter NA 0.9) as available objectives. Perhaps the reality is
different, but these objectives were quite popular and I think it
would have been a bad marketing decision to eliminate any of them.
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hi Aleksander
>
>I actually have one Leica 40X immersion objective. It could be quite old
>(it was alreay here when I arrived).
>
>It is a HCX APO L40X/0.80 W U-V-I
>
>why don't you ask for this one?
>
>best
>
>Valeria
>
>On 04/03/2011 22:46, Stephen Cody wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> G'day Aleksandr,
>>
>> Do you require this lens for confocal and or multiphoton
>>microscopy? If so you should follow George's advice.
>>
>> If you purchase a high NA 20x (or 25x) lens. Then this will
>>perform perfectly at zoom 1 with a wide field of view, and as
>>described be George zoom in 2 or 3 times and have the equivalent
>>performance of a 40 or 60x. Saving you the cost of several lenses.
>>
>> If however if you need a 40x for conventional microscopy, then you
>>may need a 40x.
>>
>> Mismatching objectives from manufactures may be possible, but is
>>not a good idea, especially if you want to use confocal microscopy
>>to optically section deep within tissue. The loss of chromatic
>>correction I think would result in very significant loss of signal
>>strength as you go deeper into tissue with confocal. Maybe not so
>>problematic if using for conventional microscopy or multiphoton
>>with NDDs.
>>
>> I wouldn't mix manufactures lenses on confocal if you were hoping
>>for good depth penetration. Which is likely the reason why you want
>>a water immersion lens in the first place.
>>
>> Cheers Steve
>>
>> Stephen H. Cody
>>
>> On 26/02/2011, at 11:43 AM, George
>>McNamara<[log in to unmask]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Leica has a 20x/1.0 NA objective lens, and, if I recall
>>>correctly, a 25x/0.95NA - if this is on a laser scanning confocal,
>>>zoom these lenses to 2x. NA is what matters.
>>>
>>> On 2/25/2011 10:10 AM, Jurkevic, Aleksandr wrote:
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>>
>>>> Hello,
>>>>
>>>> Leica doesn't currently have a 40x water immersion objective
>>>>although I heard that they have one in the pipeline. Has anyone
>>>>used a 40x water from a different manufacturer with success on
>>>>this frame?
>>>>
>>>>
>>>> Aleksandr Jurkevic, PhD
>>>> Associate Director
>>>> Molecular Cytology Core
>>>> University of Missouri-Columbia
>>>> 120 Life Science Center
>>>> 1201 E. Rollins St.
>>>> Columbia, MO 65211-7310
>>>>
>>>>
>>>
>>> --
>>>
>>>
>>> George McNamara, PhD
>>> Analytical Imaging Core Facility
>>> University of Miami
>>
>
>--
>Valeria Berno,PhD
>Microscopy Facility Manager
>MRC Centre for Regenerative Medicine
>Institute for Stem Cell Research
>University of Edinburgh
>Roger Land Building
>West Mains Road
>Edinburgh
>EH9 3JQ
>Rm 273
>Tel Office 0131 6517265
>
>
>
>The University of Edinburgh is a charitable body, registered in
>Scotland, with registration number SC005336.
--
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396
|
