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March 2011

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Subject:
Off topic - Microinjection device
From:
Pedro J Camello <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 8 Mar 2011 10:59:14 +0100
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Hi all,

 

This is a non confocal  question. Iīm trying to apply repeated
microinjections of caffeine to cells using a microinjection device from
Eppendorf (Femtojet) while determining calcium in the microscope. Our
problem is that using microinjection pipettes (tip inner diameter < 0.5 mm)
the tip gets blocked by debris from the medium (even perfusing cells). If I
use broader pipettes (letīs say 10-30 micrometers wide) the caffeine of the
tip is released prematurely. I also have a picospritzer II, but I donīt have
even the manual . 

 

I would appreciate if any body in the list with some experience with any of
these equipments contact me off-list (or on-list)

 

 

Thanks

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