*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Pedro-
Are you using the femto-tips?  This are much, much smaller in inner diameter than 0.5 mm.  They can be purchased from Eppendorf or Zeiss.  I have used them but I cannot remember which was the vendor.
Center for Microscopy & Microanalysis
Bowling Green State University
________________________________________
From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Pedro J Camello [[log in to unmask]]
Sent: Tuesday, March 08, 2011 4:59 AM
To: [log in to unmask]
Subject: Off topic - Microinjection  device

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi all,



This is a non confocal  question. I´m trying to apply repeated
microinjections of caffeine to cells using a microinjection device from
Eppendorf (Femtojet) while determining calcium in the microscope. Our
problem is that using microinjection pipettes (tip inner diameter < 0.5 mm)
the tip gets blocked by debris from the medium (even perfusing cells). If I
use broader pipettes (let´s say 10-30 micrometers wide) the caffeine of the
tip is released prematurely. I also have a picospritzer II, but I don´t have
even the manual .



I would appreciate if any body in the list with some experience with any of
these equipments contact me off-list (or on-list)





Thanks