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Hi Jason, Thank you for the good idea. Tom

On Mon, Jun 27, 2011 at 4:13 PM, Kilgore, Jason
<[log in to unmask]>wrote:

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>
> Tom, though it won't help with microscopic examination, have you considered
> using a microplate reader format?  You could use a nucleic acid stain, such
> as Hoechst 33342, and compare the signal to a standard curve generated from
> wells with known bacterial densities.
>
> Jason
>
> ** vendor association acknowledged **
>
> Jason A. Kilgore
> Technical Application Scientist
> Molecular Probes Labeling and Detection Technologies
> Cells Systems Division
>
> T 1 800 955 6288 then option 5  or  541 335 0353 . F 541 335 0238
> 29851 Willow Creek Rd . Eugene . OR . 97402-9132 . United States
> www.invitrogen.com/technicalsupport
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>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Tom Lawson
> Sent: Sunday, June 26, 2011 9:45 PM
> To: [log in to unmask]
> Subject: Concentrating bacteria cells for microscope visualization.
>
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> Dear Confocalists,
>
> I am having a few problems separating and concentrating bacteria cells for
> microscope visualization.
>
> I have a 1 ml solution in an aliquot that contains some eukaryote debris
> and
> 10 to 100 bacteria cells. After spotting the centrifuge concentrate to a
> slide-well, I can see the bacteria with a nucleic stain. But the debris in
> the concentrate makes it hard to pipette and blocks seeing the bacteria. In
> addition, the concentrate is spread over a 6 mm slide-well which makes it
> hard to locate the bacteria.
>
> I would be grateful for any suggestions.
>
> Regards,
>
> --
> Tom Lawson
> [log in to unmask]
> PhD Student,
> Macquarie University
> NSW, 2109
> Australia
>



-- 
Tom Lawson
[log in to unmask]
PhD Student,
Macquarie University
NSW, 2109
Australia