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We have the Olympus FV10i with automatic water pump, it works fine, it keeps water continuosly on the lens tip in multipoint, time lapse, z-stack acquisitions with no trouble, even combining all of them, it also controls temperature and allows CO2 input working with a external perfussion pump in 35mm dish. I am not sure it can use something different to water but I also feel there is no need
 
Gabriel OH

 

> Date: Sat, 23 Jul 2011 08:25:40 +1000
> From: [log in to unmask]
> Subject: Re: Alternative immersion media for a water immersion lens
> To: [log in to unmask]
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> 
> The other way to do it is a continual water source.
> We have the Leica 63x with computer controlled water pump (no commercial
> interest), but also use a lab-made system using a pipette, reservoir and
> pump (designed by Steve Cody).
> 
> We also use the Zeiss immersol and Cargille water RI oils but find they dry
> over time and the quality of the image deteriorates. The water pump also
> allow imaging multipoint experiments where there is considerable movement of
> the objective.
> Cheers,
> Stephen
> 
> On 23 July 2011 03:19, Farid Jalali <[log in to unmask]> wrote:
> 
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
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> >
> > Hello All. Thank you very much to everyone for your replies and insightful
> > suggestions. It never ceases to amaze me what a wonderful community this
> > is.
> > Have a great day all.
> > Cheers
> > Farid
> >
> > On Thu, Jul 21, 2011 at 9:42 PM, Farid Jalali <[log in to unmask]>
> > wrote:
> >
> > > Hello all,
> > > Is anyone aware of a suitable alternative immersion media for a water
> > > immersion lens? This lab is doing experiments at room temperature and the
> > > scope is not enclosed therefore no humidity control.
> > > Thanks very much all.
> > >
> > > Farid Jalali
> > >
> >
> 
> 
> 
> -- 
> Stephen Firth
> Manager of Advanced Optical Microscopy
> Monash Micro Imaging
> Monash University
> Room G58 Building 75
> Phone 990 55612
> Mob 0400 695 425