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The other way to do it is a continual water source.
We have the Leica 63x with computer controlled water pump (no commercial
interest), but also use a lab-made system using a pipette, reservoir and
pump (designed by Steve Cody).

We also use the Zeiss immersol and Cargille water RI oils but find they dry
over time and the quality of the image deteriorates. The water pump also
allow imaging multipoint experiments where there is considerable movement of
the objective.
Cheers,
Stephen

On 23 July 2011 03:19, Farid Jalali <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All. Thank you very much to everyone for your replies and insightful
> suggestions. It never ceases to amaze me what a wonderful community this
> is.
> Have a great day all.
> Cheers
> Farid
>
> On Thu, Jul 21, 2011 at 9:42 PM, Farid Jalali <[log in to unmask]>
> wrote:
>
> > Hello all,
> > Is anyone aware of a suitable alternative immersion media for a water
> > immersion lens? This lab is doing experiments at room temperature and the
> > scope is not enclosed therefore no humidity control.
> > Thanks very much all.
> >
> > Farid Jalali
> >
>



-- 
Stephen Firth
Manager of Advanced Optical Microscopy
Monash Micro Imaging
Monash University
Room G58 Building 75
Phone 990 55612
Mob 0400 695 425