CONFOCALMICROSCOPY Archives

July 2011

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Subject:
From:
"Jerry (Gerald) Sedgewick" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 11 Jul 2011 09:01:47 -0500
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*****
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Here's why the 2nd Annual Imaging in Research Course is being given in 
August, 2011:

* Visual data in the form of images is often 50 percent of a 
manuscript's content.  It's content is a reflection of the lab, and so 
the quality and accuracy of images is as important as the written content.

* 44 percent of cases investigated by the Office of Research Integrity 
in 2005-6 involved accusations of image fraud, compared with about 6 
percent a decade before that (1). These cases are rising, and most 
involve graduate and post-doctoral students.

* Students and staff who self-report familiarity with imaging programs 
like Photoshop may be using it improperly.

In response to this, the "2nd Annual Imaging in Research Course: Ethics, 
Acquisition, Post-Processing, Output and Quantization" is being held at 
the Stadium Village Radisson Hotel on the University of Minnesota 
campus, August 24 - 26, 2011.  Attendees can choose to attend the course 
for 2- to 3-days.  This workshop will educate those in science, medicine 
and engineering about correct techniques when acquiring, 
post-processing, and adjusting images for outputs; along with techniques 
that work for segmenting complex, biological images (for subsequent 
image analysis).

Other benefits of taking the course will likely result in:

Faster acceptance of submitted manuscripts
Authors better able to demonstrate outcomes to their target audience
Faster results from quantitation, with improved ability to segment 
desired features
Better documentation of imaging procedures
Standardization of post-processing
Learning to adjust and modify images minimally and through the objective 
use of numbers.

Jerry Sedgewick will present.  Jerry directed a core light microscopy 
and imaging facility for 15 years at the University of Minnesota, 
published 2 books on Photoshop and digital imaging, and his quantitative 
work has led to FDA approval for start up companies.

Please go to http://www.imagingandanalysis.com/seminars.html for more 
information.  There is a limit of 12 seats.

The cost ranges from $390 for 2-days to $490 for 3-days. It includes 
lunch, beverages and snacks.  Here's the summary for the days:

Day1: Ethics of digital imaging, sample preparation, calibration, best 
acquisition practices on light microscopes; setting up Photoshop, 
opening image stacks/12 bit images, rotate/crop, 
De-colorizing/colorizing fluorescent samples, merging images, 
colocalization, adjusting tones for 3D reconstructions, saving images, 
resetting pixel resolution (resampling), creating automated steps 
(macros). These functions also covered for the free programs GIMP and 
Image J (when applicable).
Day2: Post Processing II:  uneven illumination correction, color 
correction, histogram (tone) matching, correcting noise, scale bars, 
extended focus, extended dynamic range, pseudocolor, tonal adjustment, 
De-colorizing/colorizing fluorescent samples, merging images, 
colocalization, adjusting tones for 3D reconstructions, saving images, 
resetting pixel resolution (resampling), creating automated steps 
(macros).  These functions also covered for the free programs GIMP and 
Image J (when applicable).
Day 3: Segmenting in Photoshop for image analysis (quantization): 
optical density and intensity measurements, creating binary files 
through 3 methods, setting threshold at consistent value, unbiased 
sampling (stereology), automating steps, measurement in image analysis 
programs.

All the best,

Jerry Sedgewick

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