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Just a reminder about our upcoming microscopy workshop July 24-26.
Automated, Live Cell and High-Content Imaging Course: From Apoptosis to Zebrafish
July 26 through 28th, 2011 McGill University
Note the workshop is not only for people interested in RNAi or chemical screens. The equipment we are presenting and using are automated widefield microscopes and a laser scanning cytometer so any application (fixed or live) can be adapted for higher throughput. For example: Test and titrate multiple primary and secondary antibodies in as little as 10 minutes for up to 96 conditions.
Some highlights
Lectures Hands On Assays
Quantitative fluorescence GPCR Internalization
Live Cell Imaging Atophogy
Reagents talk from one of Molecular Probes bench scientists Mitotic Index
Filters talk by Semrock Cell migration
Stem Cell Assays Invasion
Custom Assays Micropattern Arrays
mRNA Imaging Toxicity
Live Cell Calcium Flux
Register by Friday July 15th at 5PM EST so we can finalize numbers.
http://mcgillatozimaging.eventbrite.com/
Please e-mail [log in to unmask] for any further information.
Complete Program:
Date
Time
Description
Tuesday,
July 26, 2011
9:00 to 12:30
Lectures
1. Welcome and Overview - Claire Brown, Assistant Professor, Director McGill Imaging Facility
2. Fluorescence, fluorescence dyes and the fluorescence microscope - Claire Brown, Assistant Professor, Director McGill Imaging Facility
3. Fluorescence Mirrors and Filters - Prashant Prabhat, Applications Scientist, Semrock
4. New Chapters from the Molecular Probes Handbook: Fluorescence labeling and Detection Strategies in Cellular Biology - Daniel W. Beacham, Senior Staff Scientist, R&D, Molecular Probes Cellular Imaging & Analysis
5. ImageXpress Micro (IXM) and Velos Instrumentation and Applications - Vipat Raksakulthai, Field Application Scientist, Molecular Devices, Inc.
13:30 to 18:30
Hands-On Exercises
1. GPCR Internalization - ImageXpress Micro
2. Mitotic Index - ImageXpress Micro
3. Mitotic Index - ImageXpress Velos
4. GPCR Inernatlization - Axiovert 1
5. Mitotic Index - LSM3
Wednesday,
July 27, 2011
9:00 to 12:10
Lectures
1. Quantitative Imaging - Judith Lacoste, Microscopy Specialist, CIAN Biology Department, Founder - MiaCellavie
2. Live Cell Imaging - Claire Brown, Assistant Professor, Director McGill Imaging Facility
3. Custom Assays for Nuclear Biology - Mohamed Kodiha, Post Doctoral Fello, McGill University
4. Autophagy and Calcium Flux Assays - Penny Tavormina, Field Application Scientist, Molecular Devices, Inc.
5. Cell Migration and Invasion Assays - Tania Villeneuve, MJS Biolynx, Inc., Platypus Distributors
13:30 to 18:30
Hands-On Exercises
1. Live Cell Autophagy Assay - ImageXpress Micro
2. Live Cell Calcium Flux Assay - ImageXpress Micro
3. Platypus Cell Migration Assay - ImageXpress Velos
4. Live Cell Autophagy Assay - Axiovert 1
5. Live Cell Calcium Flux Assay - LSM3
Thursday,
July 28, 2011
9:00 to 12:15
Lectures
1. Alternative Applications and Data Mining with AcuityXpress Software - Vipat Raksakulthai, Field Application Scientist, Molecular Devices, Inc.
2. Cell Normalization for Quantitative Cell Imaging and Analysis - Pauline Menager, Manager of Scientific Affairs, CYTOO Cell Architects
3. Advanced Applications: Stem Cells - Penny Tavormina, Field Application Scientist, Molecular Devices, Inc.
4. Defining mRNA Localization pathways in Drosophila - Eric Lécuyer, Laboratory Director - RNA Biology, Institut de Recherches Cliniques de Montréal (IRCM)
5. Open Session Q&A
13:30 to 18:30
Hands-On Exercises
1. CYTOO Micropatterned Plate - Streamline Your Assay - ImageXpress Micro
2. Platypus Invasion Assay - ImageXpress Micro
3. Toxicity Assay and Q&A session - ImageXpress Velos
4. CYTOO Micropatterned Plate - Axiovert 1
5. Toxicity Assay - Laser Scanner Confocal
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