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Hi Emma

I am very happy with Nikon's NIS-Elements software, but just wanted to
second Graham Wright's & Louis Kerr's input - the stackreg algorithm works
a charm. I often use its modified version MultiStackReg, aligning one
channel and applying the same translation to the other(s). Very useful in
case of moving cells, stage drift etc.

http://www.stanford.edu/~bbusse/work/downloads.html

Speaking of which: Many thanks to Thierry Thévenaz & Michael Unser and Brad
Busse & Steven Smith for these fabulous open-source tools!

Philippe


Philippe Laissue, PhD
Bioimaging Facility, University of Essex, UK