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Hello -

I was wondering what people's experience is with the far red FP variants - mPlum
or mRasberry
Roger Tsien's Nat. Methods paper from 2005 says mPlum is better. Have people
successfully been using this FP?
Would be great to hear recommendations/things to consider.
And if one would also want to image eGFP and another FP in between, what would
people recommend for the optimal combination of spectral separation but also
fluor brightness/stability? 

I have a core user who is aiming to express all three - I realize this is a
difficult task, just for cell health and transfection, but was hoping for FP
choice recommendations. 

Thank you for any feedback -
Feel free to contact me off list.
Lisa


---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy Core
Dana Farber Cancer Institute
Boston, MA 02215
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