***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Axel, There seem to be a number of options at your disposal that are based out of ImageJ. Try looking here: http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:frap_analysis:start and here (at the bottom): http://www.macbiophotonics.ca/imagej/intensity_vs_time_ana.htm There's even a nice "how to" JOVE video: http://www.jove.com/video/2568/fluorescence-recovery-after-photobleaching-frap-of-fluorescence-tagged-proteins-in-dendritic-spines-of-cultured-hippocampal-neurons It seems to me that even ImageJ's basic intensity vs time tools and the built in curve fitter could be used to construct a simple and customizable FRAP analysis tool for your particular sample type if you wanted. Cheers John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada www.spectral.ca On 2011-11-22, at 4:14 AM, Axel Kurt Preuss (IMCB) wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > > Hi everybody > Would anybody know a freeware or online software tool, simple to use, which calculates the half time of FRAP curves > > Thanks > Axel > > > Note: This message may contain confidential information. If this Email/Fax has been sent to you by mistake, please notify the sender and delete it immediately. Thank you.
