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Hi

I'm not sure I understand why you have to notch out the 491 from the lamp? I am assuming you want both the laser and the lamp light travelling the same route, and you are therefore just combining the beams?
The cheapest option is a broad spectrum beam splitter (a % silvered mirror) which will lose some of your light but you probably have excess anyway.
Otherwise you think you want a prism to combine the paths.

Sorry if I have misunderstood

Regards
Kenton


Dr Kenton Arkill
MVRL
Southwell St.
Bristol
BS2 8EJ
Office Tel. 0117 92 88367

THE BIG BANG THEORY: THE PHYSICS IS THEORETICAL BUT THE FUN IS REAL

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Christophe Leterrier
Sent: 30 November 2011 13:33
To: [log in to unmask]
Subject: notch dichroïc filter: does it exist?

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Hi,  

On a widefield/FRAP setup, I want to combine a laser at 491 nm (fiber coupling) with an HBO xenon lamp (direct mount) on the back of my scope. The way things are arranged, I need to put a 45° dichroic mirror so that the lamp light will go through the mirror into the scope and the laser light will be reflected into the scope. So I'm looking for a dichroic mirror that would transmit everything (from 400 to 750) but a small window arround 491 nm. The window has to be narrow so that I don't loose too much GFP excitation light from the lamp. Is that kind of dichroïc available? I could'nt find one browsing Chroma or Semrock offers. Of course there is the option of having a custom one made, but I was wondering if someone had a link to an existing offer.

On a related note, I found lots of notch filters but not a lot of notch dichroics. What is the difference between a simple filter and a dichroic? What happens to the blocked light in the case of a filter, isn't it also reflected like for a dichroic?

Thanks for your help,

Christophe

--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
http://www.cleterrier.net