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November 2011

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Subject:
5th colour?
From:
Simon Walker <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 4 Nov 2011 11:36:50 -0500
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Hello,
Has anyone successfully imaged 5 colours in the same sample with their 
confocal?  We have a user who is keen to do this.  They are already using 
DAPI, AF488, AF555 & AF647 so if there's something that maybe excites in the 
blue and emits in the red/far red?  Or if that's not possible a completely 
different set of 5.  We have the usual range of excitation wavelengths from 
405 up to 635 nm.
Thanks,
Simon

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