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Dear Colleagues,

We're trying to image myelin and face a strange problem.
a. Over the time course the DiD lipophilic dye is getting brighter.
When observing the sample after LSM with mercury light an overly
bright square can be visualized.
b. Signal in green channel from myelin particles is increasing with
time as well. Since DiD is supposed to fluoresce in 650 - 750, this is
strange. Non-labeled myelin didn't seem to have the same signal. This
can't be a cross-talk - we are using spectral separation, sequential
scanning (FV1000). Same effect observed on Zeiss 710.

Any suggestions?

Thank you in advance,
Vladimir

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Vladimir Ghukasyan, Ph.D.
Confocal and Multiphoton Imaging Facility
Neuroscience Center
University of North Carolina

115 Mason Farm Rd., Bld. 245, Rm. 7109F
Chapel Hill 27599-7250
NC

Tel.: (919) 966 5807
Fax: (919) 966 9605